Red areas show the distribution of Japanese Enecphalitis in Asia 1970-1998
Find information on thousands of medical conditions and prescription drugs.

Japanese encephalitis

Japanese Encephalitis (日本脳炎 Nihon-nōen)is a disease caused by the mosquito borne Japanese Encephalitis Virus. Japanese Encephalitis Virus is a virus from the family Flaviviridae. Domestic pigs and wild birds are reservoirs of the virus, transmission to humans may cause severe symptoms. One of the most important vectors of this disease is the mosquito Culex tritaeniorhynchus. This disease usually affects people in South East Asia and the Far East. more...

Home
Diseases
A
B
C
D
E
F
G
H
I
J
Jackson-Weiss syndrome
Jacobs syndrome
Jacobsen syndrome
Japanese encephalitis
Johnson Munson syndrome
Joubert syndrome
Jumping Frenchmen of Maine
Juvenile dermatomyositis
Juvenile rheumatoid...
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Medicines

Severe rigours mark the onset of this disease in humans. Fever, headache and malaise are other non-specific symptoms of this disease which may last for a period between 1 to 6 days. Signs which develop during the acute encephalitic stage include neck rigidity, cachexia, hemiparesis, convulsions and a raised body temperature between 38 and 41 degrees Celsius. Mental retardation developed from this disease usually leads to coma. Mortality of this disease varies but is generally much higher in children. Life-long neurological defects such as deafness, emotional lability and hemiparesis may occur in those who have had Central Nervous System involvement.

The diagnosis of this disease is made by detecting antibodies in serum and CSF (cerebrospinal fluid) by IgM capture ELISA. A vaccine is available but anti-virals are usually ineffective unless administed within hours of being infected, hence treatment is mainly supportive. Japanese Encephalitis is not transmitted between humans. Infection with JEV confers life-long immunity.

Japanese encephalitis is the leading cause of viral encephalitis in Asia with 30,000–50,000 cases reported annually, case-fatality rates range from 0.3% to 60%. Rare outbreaks in U.S. territories in Western Pacific have occurred. Residents of rural areas in endemic locations are at highest risk, Japanese encephalitis does not usually occur in urban areas. Countries which have had major epidemics in the past, but which have controlled the disease primarily by vaccination, include China, Korea, Japan, Taiwan and Thailand. Other countries that still have periodic epidemics include Vietnam, Cambodia, Myanmar, India, Nepal, and Malaysia. Japanese encephalitis has been reported on the Torres Strait Islands and two fatal cases were reported in mainland northern Australia in 1998, the maintanance of spread of the virus in Australia is of particular concern to Australian health officials due to the unplanned introduction of Culex gelidus, a potential vector of the virus, from Asia.

As of September, 2005, a Japanese Encephalitis outbreak is ongoing in India.

Read more at Wikipedia.org


[List your site here Free!]


Japanese encephalitis virus in meningitis patients, Japan
From Emerging Infectious Diseases, 3/1/05 by Masaru Kuwayama

Cerebrospinal fluid specimens from 57 patients diagnosed with meningitis were tested for Japanese encephalitis virus, Total RNA was extracted from the specimens and amplified. Two products had highest homology with Nakayama strain and 2 with Ishikawa strain, Results suggest that Japanese encephalitis virus causes some aseptic meningitis in Japan.

**********

Japanese encephalitis virus is one of the leading causes of epidemic encephalitis worldwide; 35,000-50,000 cases are reported each year with 10,000 deaths (1). Before 1960, >1,000 Japanese encephalitis cases were reported annually in Japan. Because the Japanese encephalitis immunization program was introduced [approximately equal to] 30 years ago, and because of changes in rice farming, the annual number of Japanese encephalitis cases has decreased dramatically. Fewer than 10 Japanese encephalitis cases have been reported annually since 1990 (2). During the past 10 years, only 3 Japanese encephalitis cases have been reported in Hiroshima prefecture, the western part of the main island of Japan (Figure 1); all of these cases were reported in 2002 (2,3).

[FIGURE 1 OMITTED]

Japanese encephalitis virus causes meningitis as well as encephalitis (4). However, physicians tend not to list Japanese encephalitis virus as a cause of meningitis. In this study, we examined cerebrospinal fluid (CSF) specimens from patients with aseptic meningitis for Japanese encephalitis virus genome.

The Study

CSF specimens were obtained for diagnostic purposes from 170 patients with a clinical diagnosis of aseptic meningitis from August to October in each year from 1999 to 2002, in Hiroshima prefecture. These CSF specimens were sent to the Division of Microbiology II, Hiroshima Prefectural Institute of Health and Environment, for virologic examination. Viruses were isolated from 112 of 170 CSF specimens in cell cultures by using Vero cells, BGM cells, FL cells, Hep2 cells, and RD 18S cells. Enteroviruses and mumps viruses were isolated from 96 and 16 CSF specimens, respectively (see online Appendix Table, available from http://www.cdc.gov/ncidod/eid/04-0285_app .htm). Thus, etiologic agents were not determined for 58 meningitis cases. CSF specimens from 57 of these 58 cases were used to detect Japanese encephalitis virus genome. Whether the 57 patients had been vaccinated for Japanese encephalitis virus was not known.

Total RNA was extracted from CSF by using ISOGENLS (Nippon Gene, Tokyo, Japan). The RNA pellet was resuspended in DEPC Treated Water (Invitrogen Corp., Carlsbad, CA, USA). The RNA was reverse transcribed and amplified by using polymerase chain reaction (PCR) with AMV Reverse Transcriptase XL (Life Sciences Inc., St. Petersburg, Florida, USA.) and Tth DNA Polymerase (Toyobo Co., Ltd., Osaka, Japan) with the primer pair reported by Morita et al. (5). The PCR product was nested PCR-amplified by TaKaRa EX Taq (Takara Bio Inc., Otsu, Japan), with the primer pairs for E gene region reported by Kimura et al. (6); JEN ATC GTG GTT GGG AGG GGA GA(1147-1166)_JENR_AGC ACA CCT CCT GTG GCT AA(1472-1453). The DNA amplicons were separated by electrophoresis on 1.5% (wt/vol) agarose gel, followed by staining with ethidium bromide (1 ktg/mL). The target band detected by electrophoresis was purified by using the Qiagen gel extraction kit (QIAGEN Inc., Valencia, CA, USA). Purified cDNA was directly sequenced in both directions by using the sense and the antisense primers JEN and JENR. The samples that could not be sequenced using these primers were reverse transcribed and PCR-amplified, nested PCR-amplified, and directly sequenced by using other primers designed to amplify the Japanese encephalitis virus E gene region; RT-PCR JEen37s-first: AAG GAG CCA GTG GAG CCA CTT, JEen329c-first: TTC CCG AAA AGT CCA CAT CC, nested PCR and sequence JEen98s-second: CAT GGC AAA CGA CAA ACC AAC, JEen301c-second: CAG TRA AGC CTT GTT TGC ACA C. The samples that could not be directly sequenced by using the second primer sets were amplified with nested PCR and sequenced by using JEen98s-second and JEen271r-inner (RGT RAA GCC TTG TTT GCA CAC).

Electrophoresis demonstrated the band with expected size of 326 bp in 4 of 57 PCR products (Figure 1). Two (numbers 1 and 2) of these products were sequenced on 326 bp and 247 bp, respectively, and the highest homology was with Japanese encephalitis virus, Nakayama strain (genotype III). PCR products 3 and 4 were sequenced on 121 bp and 187 bp by using other primers, and the highest homology was with Japanese encephalitis virus, Ishikawa strain (genotype I) (Table 1).

Patients 1-4 lived in Hiroshima prefecture (Figure 2). Patient 1 was a 3-year-old boy and patient 4 was a 6-year-old girl (Table 2). They became sick in early August 2000. Patient 2 was a 2-year-old girl who became sick in late August 2000. Patient 3 was a 4-year-old boy who got sick in mid-September 2000. CSF samples were obtained 2-3 days after the onset of illness. All 4 patients had symptoms characteristic of meningitis and were clinically diagnosed with meningitis.

[FIGURE 2 OMITTED]

Conclusions

Enteroviruses are the most frequent cause of aseptic meningitis in summer and autumn in Japan (online Appendix Table). In the CSF samples from these 4 patients, however, enteroviruses were not found. Watt et al. reported that in Thailand, 14% of adult patients with acute, undifferentiated fever had a diagnosis of Japanese encephalitis virus infection because anti-Japanese encephalitis virus immunoglobulin (Ig) M antibodies were found (10). Many physicians in Japan consider Japanese encephalitis virus to be mainly associated with encephalitis, and examination for the virus is not usually conducted for patients with meningitis or undifferentiated fevers.

Three Japanese encephalitis cases occurred in 2002 for the first time in 12 years, but anti-Japanese encephalitis virus IgM antibodies were detected in porcine serum samples every year in Hiroshima prefecture (data not shown). So we expected that Japanese encephalitis virus might be associated with aseptic meningitis, and CSF specimens from 57 cases during the past 5 years (1998-2002) had been stored at -30[degrees]C in Hiroshima Prefectural Institute of Health and Environment. Those specimens were used to detect Japanese encephalitis virus genome retrospectively. Four of 57 CSF samples were Japanese encephalitis virus genome-positive by nested PCR. We may find more febrile or meningitis cases caused by Japanese encephalitis virus if we routinely conduct serologic or molecular diagnostic tests for Japanese encephalitis virus. CSF samples were from infants and children. Most Japanese encephalitis patients in Japan are [greater than or equal to]55 years of age. Japanese encephalitis meningitis may also occur among elderly meningitis patients. The Japanese encephalitis vaccination coverage of children has been decreasing in Japan recently; <10 Japanese encephalitis cases are reported annually. If the general population recognizes that Japanese encephalitis virus causes aseptic meningitis at a higher rate than expected, the percentage of Japanese encephalitis vaccination may increase, and the emergence of Japanese encephalitis will be prevented. In 2000, anti-Japanese encephalitis virus IgM antibody was first detected in porcine serum samples in late July, in Hiroshima prefecture (data not shown). This finding suggests that Japanese encephalitis virus was active when these 4 meningitis cases occurred. Further, we found evidence that Japanese encephalitis viruses belonging to genotypes I and III were active in Hiroshima prefecture (data not shown). The results of this study suggest that Japanese encephalitis virus should be considered in the differential diagnosis of aseptic meningitis in areas where Japanese encephalitis is endemic or epidemic.

Acknowledgments

We thank physicians and pediatricians in the hospitals and clinics in Hiroshima prefecture for providing us with CSF samples.

This study was in part supported by the grant for the Research on Emerging and Re-emerging Infections Diseases from the ministry of Health, Labour and Welfare of Japan.

References

(1.) Tsai TF. New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO/CVI meeting, Bangkok, Thailand, 13-15 October 1998. Vaccine. 2000;18 (Suppl. 2):1-25.

(2.) Japanese encephalitis, Japan, 1992-2002. Infectious Agents Surveillance Report. 2003; 24:149-50.

(3.) Japanese encephalitis, 1991-1998. Infectious Agents Surveillance Report. 1999;20:185-6.

(4.) Burke DS, Monath TP. Flaviviruses In: Knipe DM, Howley PM., editors. Field's virology. Philadelphia: Lippincott Williams and Wilkins; 2001.

(5.) Morita K, Tanaka M, Igarashi A. Rapid identification of dengue virus serotypes by using polymerase chain reaction. J Clin Microbiol. 1991;29:2107-10.

(6.) Kimura A, Kyushi T, Yamasaki K. Detection of Japanese encephalitis virus genome from mosquito by PCR method. Bulletin of Osaka Prefectural Institute of Public Health (Japanese). 1992; 30:59-64.

(7.) Takegami T, Ishak H, Miyamoto C, Shirai Y, Kamimura K. Isolation and molecular comparison of Japanese encephalitis virus in Ishikawa, Japan. Jpn J Infect Dis. 2000;53:178-9.

(8.) Chen WR, Tesh RB, Rico-Hess R. Genetic variation of Japanese encephalitis virus in nature. J Gen Virol. 1990;71:2915-22.

(9.) Chen WR, Rico-Hess R, Tesh RB. A new genotype of Japanese encephalitis virus from Indonesia. Am J Trop Med Hyg. 1992;47:61-9.

(10.) Watt G, Jongsakul K. Acute undifferentiated fever caused by infection with Japanese encephalitis virus. Am J Trop Med Hyg. 2003;68:704-6.

Dr. Kuwayama is an infectious disease specialist and virologist. He is a vice-chief researcher at Division of Microbiology II, Hiroshima Prefectural Institute of Health and Environment, Hiroshima City, Hiroshima Prefecture, Japan. His research interests include emerging and reemerging viral infections, viral epidemiology, and zoonoses.

Address for correspondence: Masaru Kuwayama, Division of Microbiology II, Hiroshima Prefectural Institute of Health and Environment, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan; fax: +81-82-252-8642; email: kuwasan928@yacht.ocn.ne.jp

Masaru Kuwayama, * Mikako Ito, ([dagger]) Shinichi Takao, * Yukie Shimazu, * Shinji Fukuda, * Kazuo Miyazaki, * Ichiro Kurane, ([dagger]) and Tomohiko Takasaki ([dagger])

* Hiroshima Prefectural Institute of Health and Environment, Hiroshima, Japan; and ([dagger]) National Institute of infectious Diseases, Tokyo, Japan

COPYRIGHT 2005 U.S. National Center for Infectious Diseases
COPYRIGHT 2005 Gale Group

Return to Japanese encephalitis
Home Contact Resources Exchange Links ebay