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Brucellosis (Undulant fever or Malta fever) is an infectious disease caused by the Brucella bacteria, which induces inconstant fevers, sweating, weakness, anorexia, headaches, depression and muscular and bodily pain. The popular name of the condition is originated due to the inconstance (or undulance) of the fever, which raises and falls constantly. Brucellosis is named after its researcher David Bruce. more...

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Bacterial food poisoning
Bacterial meningitis
Bacterial pneumonia
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Bardet-Biedl syndrome
Bardet-Biedl syndrome
Bardet-Biedl syndrome
Bardet-Biedl syndrome
Barrett syndrome
Barth syndrome
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Becker's muscular dystrophy
Becker's nevus
Behcet syndrome
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Bloom syndrome
Blue diaper syndrome
Blue rubber bleb nevus
Body dysmorphic disorder
Bourneville's disease
Bowen's disease
Brachydactyly type a1
Bright's disease
Brittle bone disease
Bronchiolotis obliterans...
Bronchopulmonary dysplasia
Brown-Sequard syndrome
Brugada syndrome
Bubonic plague
Budd-Chiari syndrome
Buerger's disease
Bulimia nervosa
Bullous pemphigoid
Burkitt's lymphoma
Cavernous angioma

The disease is transmitted either through contaminated or untreated milk (and its derivates) or through direct contact with infected animals, which may include sheep, pigs, goats, cattle, camels, bison, and other ruminants. This also includes contact with their carcasses.

In animals this disease is also known as contagious abortion and infectious abortion. In 1897 Danish veterinarian Bernhard Bang isolated Brucella abortus as the agent and the additional name Bang's disease was assigned. In modern usage "Bang's disease" is often corrupted to just "bangs" when ranchers discuss the disease or vaccine.

The incubation period of brucellosis is, usually, of one to three weeks, but some rare instances may take several months to surface. The symptoms are like those associated with many other febrile diseases, but with emphasis on muscular pain and sweating. The duration of the disease can vary from a few weeks to many months.

The disease's sequelae are highly variable and may include granulomatous hepatitis, arthritis, spondylitis, anemia, leukopenia, thrombocytopenia, meningitis, uveitis, optic neuritis and endocarditis.

Antibiotics like tetracyclins, chloramphenicol, rifampin and the aminoglycosides streptomycin and gentamicin are effective against Brucella bacteria. However, the use of more than one antibiotic is needed for several weeks, due to the fact that the bacteria incubates within cells.

The main way of preventing Brucellosis is the proper pasteurization of all milk that is to be ingested by human beings, either in its pure form or as a derivate, such as cheese.


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Presumptive diagnosis of brucellosis from contaminated blood cultures in an area of endemicity
From British Journal of Biomedical Science, 1/1/04 by Rich, M

Brucellosis is a typical zoonosis that is endemic in large areas of the Middle East,1 and, despite improved medical care, it remains a major cause of morbidity in the area. In Saudi Arabia, Brucella sp. is one of the most common isolates from blood culture samples, and 75 examples were isolated from a total of 1903 positive blood culture specimens (4%) at the King Fahad National Guard Hospital during 2000.

Institution-specific blood culture contamination rates vary from 1% to more than 6%,2 and in this institution the contamination rate for the total number of blood cultures collected during 2000 was 3.8%. Owing to the high isolation rate and the fastidious nature of this organism, it is possible that some of the contaminated blood cultures may mask the presence of Brucella sp. when these specimens are subcultured on routine bacteriological culture media. A previous study has showed that a direct urease test on positive blood cultures that are suggestive of Brucella sp. is useful in the presumptive diagnosis of brucellosis.3

The aim of this study is to explore the utility of the direct urease test to detect the presence of Brucella sp. in blood culture vials that also contain coagulase-negative staphylococci, a common contaminant.

Simulated blood cultures were prepared using a 0.5 Macfarland standard suspension of Staphylococcus epidermidis (ATCC 12228) and similar suspensions of 50 separate patient isolates of Brucella sp. isolated during 2000. Each suspension was further diluted to give a final concentration of 10-100 colony-forming units (CFU)/mL and this was used as the working dilution. Each of 50 BACTEC aerobic blood culture vials were inoculated with 5 mL of the working dilution of both coagulase-negative staphylococci and one of the brucella suspensions. Three control vials were also prepared: one containing an isolate of Brucella sp., one containing a test strain of coagulase-negative staphylococci and one with no organisms added. Each blood culture vial was then loaded onto the BACTEC 9240 automated blood culture machine.

All the test vials automatically flagged as positive after 18-48 h incubation. The control vial containing the coagulase-negative staphylococci alone flagged as positive after 43 h and the control vial containing Brucella sp. alone flagged as positive after 83 h. The uninoculated blood culture vial remained negative at seven days.

All specimens were Gram-stained and subcultured onto a sheep blood agar plate and a urea slant, both of which were incubated at 37°C in an aerobic atmosphere. The original blood culture vials were retained and placed in a 37°C incubator.

Gram-stained preparations from all the culture vials showed Gram-positive cocci in clusters, indicative of Staphylococcus sp. The urea slants were checked after 4, 6, 8 and 24 h. After 4 h, 44 (88%) of the 50 urea slants were positive. After 6 h, a further three urea slants were positive, giving a total of 47 (94%) and one more slant was positive at 24 h, giving a grand total of 48 (96%). The control vial inoculated with Brucella sp. gave a positive urease test after 4 h. The control vial inoculated with S. epidermidis and the uninoculated control vial both remained urease-negative at 24 h.

The retained blood culture vials were subcultured onto fresh urea slants after four, five and seven days' incubation. A total of 49 (98%) out of the 50 urea slants inoculated after four days' incubation of the original vial gave a positive reaction within 4 h. Control vials gave the same reaction as previously described. This profile did not change when repeated after five and seven days' incubation.

Coagulase-negative staphylococci is the most frequently isolated blood culture contaminant.4-6 The problem of pseudobacteraemia and the clinical and financial consequences of unnecessary treatment are well documented.7 In Saudi Arabia, where brucellosis is endemic, there is the added possibility that brucella bacteraemia may be masked by the presence of contaminating organisms in blood cultures.

The present study showed that a simple biochemical test used directly on blood cultures that flag positive and show what may prove to be contaminating organisms on primary Gram-stain (Gram-positive cocci in clusters) can detect underlying, coexistent Brucella sp. This direct test appeared to be more useful if used on retained blood culture vials, once the result of the culture is known, and would ensure that test is only applied to those organisms highlighted as probable contaminants. In the present study, 98% Brucella sp. masked on subculture by coagulase-negative staphylococci gave a positive urease test after 4 h on blood culture vials retained for four days.

Although it is possible that a urease-positive organism may contaminate the blood culture vial, it is unlikely that one would do so that produces such potent urease activity as to give a positive reaction within 4 h. Thus, it is concluded that a direct urease test on blood cultures containing coagulase-negative staphylococci that may mask the presence of co-existing Brucella sp. may prove a useful tool in the presumptive diagnosis of brucellosis from contaminated blood cultures in an area of endemicity.


1 Corbel MJ. Brucellosis: an overview. Emerg Inject Dis 1997; 3: 213-21.

2 Schifman RB, Strand CL, Meier FA, Howanitz PJ. Blood culture contamination: a College of American Pathologists' Q-probes study involving 640 institutions and 497,134 specimens from adult patients. Arch Pathol Lab Med 1998; 122: 216-21.

3 Rich M, Bannatyne RM, Memish ZA. Direct urease test on BACTEC blood cultures: Early presumptive diagnosis of brucellosis in an area of endemicity. J Clin Microbiol 2000; 38: 1706.

4 Kim SD, McDonald LC, Jarvis WR et al. Determining the significance of coagulase-negative staphylococci isolated from blood cultures at a community hospital: a role for species and strain identification. Infect Control Hosp Epidemiol 2000; 21: 213-7.

5 Souvenir D, Anderson DE, Palpant S et al. Blood cultures positive for coagulase-negative staphylococci: antisepsis, pseudobacteremia, and therapy of patients. J Clin Micobiol 1998; 36: 1923-6.

6 Thylefors JD, Harbarth S, Pittet D. Increasing bacteremia due to coagulase-negative staphylococci: fiction or reality? Infect Control Hosp Epidemiol 1998; 19: 581-9.

7 Freney J, Kloos WE, Hajek V et al. Recommended minimal standards for description of new staphylococcal species. Subcommittee on the taxonomy of staphylococci and streptococci of the International Committee on Systematic Bacteriology. Int J Syst Bacteriol 1999; 49: 489-502.

M. RICH* and Z. A. MEMISH[dagger]

* IDEXX Laboratories, Grange House, Wetherby, West Yorkshire, UK; and [dagger] Departments of Infection Prevention and Control and Medicine, King Abdulaziz Medical City - King Fahad National Guard Hospital, Riyadh, Saudi Arabia

Correspondence to: Dr Zind A. Memish

Infections Diseases Division, Department of Internal Medicine, King Abdulaziz Medical City - King Fahad National Guard Hospital, P.O. Box 22490, Riyadh 11426, Saudi Arabia.


Copyright Step Publishing Ltd. 2004
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