Carbenicillin

To the Editor:

I wish to make corrections of typographical errors in the METHODS section of our article published in the AJRCCM (1).

The final concentrations of antibiotics in the selective media when plating lung homogenates on 7H10 medium were as follows:

* cycloheximide, 50 µg/ml (not 10 as reported)

* polymyxin B, 200 U/ml (not 200,000 as reported)

* carbenicillin, 50 µg/ml as reported

* trimethoprim, 20 µg/ml as reported.

In the subsection of METHODS entitled "ASSESSMENT OF TREATMENT EFFICACY," the last sentence should read "CFU counts were performed as previously described (15), except that plates for lung homogenates were supplemented with cycloheximide (50 µg/ml), polymyxin B (200 U/ml), carbenicillin (50 µg/ml), and trimethoprim (20 µg/ml) to prevent contamination (adapted from Mitchison and coworkers [29])."

Conflict of Interest Statement: J.H.G. does not have a financial relationship with a commercial entity that has an interest in the subject of this manuscript.

References

1. Nuermberger EL, Yoshimatsu T, Tyasgi S, O'Brien RJ, Vernon AN, Chaisson RE, Bishai WR, Grosset JH. Moxifloxacin-containing regimen greatly reduces time to culture conversion in murine tuberculosis. Am J Respir Crit Care Med 2004;169:421-426.

JACQUES H. GROSSET

Johns Hopkins University

Baltimore, Maryland

Copyright American Thoracic Society Apr 1, 2005
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