Porphyria

Abstract

The essential oil of Phoebe porphyria (Griseb.) Mez. (Lauraceae) collected from northwestern Argentina (Yungas area) was analyzed by GC/MS. Twenty-five components were characterized, representing 87.0% of the total components detected. 1,8-Cineole (10.5%), [beta]-caryophyllene (19.3%) and spathulenol (17.1%) were the major constituents of the oil, which was obtained in 0.15% yield.

Key Word Index

Phoebe porphyria, Lauraceae, essential oil composition, 1,8-cineole, [beta]-caryophyllene, spathulenol.

Plant Name

Phoebe porphyria (Griseb.) Mez., Lauraceae. Common name 'laurel de la falda' (1).

Source

The plant was collected from Tartagal, province of Salta, in October 2000. Voucher specimens have been deposited in the Herbarium of the Museo Botanico de Cordoba (CORD).

Plant Part

Air-dried aerial parts were subjected to hydrodistillation for 3 h using a Clevenger-type apparatus to produce oil in 0.15% yield.

Previous Work

To the best of our knowledge, nothing is known about the chemical composition of P. porphyria oil. However, ocoteine has been identified in this species (2). In addition, an analysis of P. porosa oil has boon published (3).

Present Work

Gas chromatography: The oil was analyzed with a Shirnadzu GC-RIA gas Chromatograph equipped with a fused silica column (30 m x 0.25 mm) coated with DB-5. The temperature of the column was programmed from 60°-240°C at 4°C/min. The injector and detector temperatures were at 250°C. The gas earner was He, at a flow rate of 1 mL/min. Peak areas were measured by electronic integration. The relative amounts of the individual components are based on the peak areas obtained, without FID response factor correction. Programmed temperature retention indices of the compounds were determined relative to n-alkanes. GC analysis was still performed using a column Supelcowax-10 with the same conditions as described above. Gas chmmatography/mass spectrometry analyses were performed on a Perkin Elmer Q-910 using a 30 m x 0.25 mm capillary column coated with DB-5. The temperature of the column and the injector were the same as those of GC. The carrier gas was He, at a flow rate of mL/min. Mass spectra were recorded at 70 eV The oil components were identified by comparison of their retention indices, mass spectra with those of authentic samples, by peak enrichment, mass spectra library of National Institute of Standards and Technology (NIST 3.0), Adams's library (4), and our mass spectra library, which contains references mass spectra and retention indices of volatile compounds. GC/MS analysis was still performed using a column Supelcowax 10 with the same conditions as describe above. The chemical components identified in P. porphyria oil are listed in Table I. The oil was found to be rich in the sesquiterpenoid compounds spathulenol and [beta]-caryophyllene as well as 1,8-cineole.

Acknowledgments

The authors thank CONICOR, SECyT-UNC and PROYUNGAS (LIEY) for financial support. We also thank Biol. N. von Muller for technical assistance.

References

1. L. Parodi and M. Dimitri, Enciclopedia Argentina de Agricultura y Jardineria. p. 398. Bs. As. Acme. (1959).

2. F. Baralle, A. Busch, MJ Vernengo and AM Kuck, Isolation of ocoteine from Nectandra saligna and Phoebe porfiria. Lloydia, 35, 300-302 (1972).

3. T. Reynolds and G. Kite, Volatile constituents of Phoebe porosa Mez. J. Essent. Oil Res. 7, 415-418 (1995).

4. R.P. Adams, Identification of essential oil components by Gas Chromatography/Mass Spectroscopy. Allured Publ. Corp. Carol Stream, IL. USA (1995).

Maria L. Lopez, Maria P. Zunino and Julio A. Zygadlo*

Institulo Multidisciplinario de Biologia Vegetal, Facultad de Ciencias Exactas, Fisicas y Naturales, Universidad Nacional de Cordoba, Avenida Velez Sarsfield 1600, 5000 Cordoba, Argentina

Abel G. Lopez, Enrique I. Lucini and Silvina M. Faillaci

Inslituto de Ciencia y Tecnologia de los Alimentos, Facultad de Ciencias Exactas, Fisicas y Naturelles, Universidad National de Cordoba, Avenida Velez Sarsfield 1600, 5000 Cordoba, Argentina

"Address for correspondence Received: January 2001

Revised: February 2001

1041-2905/04/0002-0129S6.00/0-© 2003 Allured Publishing Corp. Accepted: May 2001

Copyright Allured Publishing Corporation Mar/Apr 2004
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