Background: Exogenous administration of selected growth factors exert gut-trophic effects and may improve mucosal redox status. However, little comparative data on effects of specific growth factors after small bowel resection (SBR) are available. We hypothesized that an analog of glucagon-like peptide-2 (GLP-2) would exert superior gut-trophic effects after SBR than either growth hormone (GH) or keratinocyte growth factor (KGF) and that KGF would have the more potent effect on mucosal glutathione (GSH) redox status.
Methods: Thirty-seven male rats underwent small bowel transeclion with s.c. saline (TxS; n=7) or 80% SBR with either s.c. saline (RxS, n=7), GLP-2 (RxGLP-2, 0.2mg/kg/d, n=8), KGF (RxKGF, 3.0mg/kg/d, n=7) or GH (RxGH, 3.0mg/kg/d, n=8). all rats were pair-fed standard diet to intake of RxS rats. After 7 days, DNA content was measured in full-thickness jejunum, ileum, and colon. Villus height in small bowel segments and crypt depth in all gut segments were measured and total mucosal thickness calculated. Mucosal expression of goblet cells and the cyloprotective trefoil peptide TFF3 were determined by morphology and Western blotting, respectively. The ratio of mucosal GSH to glutathione disulfide (GSSG) was determined as an index of redox status.
Results: SBR (RxS) increased small bowel and colonie mucosal growth indices versus control (TxS) and oxidized ileal and colonie mucosal GSFI redox (Tables 1, 2, and 3). SBR did not affect gut goblet cell number or TFF3 expression. GLP-2 increased jejunal villus height, total mucosal thickness and DNA content compared to RxS, RxGH or RxKGF (Tables 1 and 2). Growth indices in ileum were similar in the GLP-2, GH and KGF groups, while in colon, GH and KGF modestly increased mucosal crypt depth vs RxS (Tables 1 and 2). hi jejunual mucosa, GLP-2 upregulated the GSH/GSSG ratio vs RxS, while KGF had an intermediate effect, hi both ileum and colon, GLP-2 (but not GH or KGF), prevented the SBR-induced decline in the mucosal GSF1/GSSG ratio (Table 3). Goblet cell number and TFF 3 expression in small bowel and colon were unaffected by GLP-2 or GH, but were markedly upregulated by KGF in all gut segments.
Conclusions: GLP-2 exerts superior effects on adaptive jejunal growth and small bowel and colonie GSH redox status compared to GH or KGF after massive SBR in rats. Both GFI and KGF increase colonie mucosal growth in this model. KGF has potent trophic effects on gut mucosal goblet cells and TFF3 expression after SBR that are not shared by GLP-2 or GH.
N. Washizawa, MD1; L. H. Gu, MD1; L. Gu1; K. P. Openo, MPH2; D. P. Jones, MD2; T. R. Ziegler, MD1; 1 Department of Medicine, Emory University, Atlanta, Georgia; 2Biochemistry, Emory University, Atlanta, Georgia.
Copyright American Society for Parenteral and Enteral Nutrition Jan/Feb 2004
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